Caldesmon antibody

• Catalog Number: orb1151813
• Category: Antibodies
• Description: Mouse monoclonal antibody to Caldesmon
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rCALD1/820
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human CALD1 protein was used as the immunogen for the recombinant Caldesmon antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Caldesmon antibody is available for research use only.
• UniProt ID: Q05682
• Storage: Aliquot the recombinant Caldesmon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant Caldesmon antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human ovary tissue lysate using recombinant Caldesmon antibody (clone rCALD1/820). Predicted molecular weight ~93 kDa, can be observed at 70-80 kDa (non muscle tissue) and 120-150 kDa (smooth muscle).

IHC staining of FFPE human leiomyosarcoma tissue with recombinant Caldesmon antibody (clone rCALD1/820). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Caldesmon antibody (clone rCALD1/820) as confirmation of integrity and purity.