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Catalog Number | orb1274240 |
---|---|
Category | Antibodies |
Description | CALD1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | CALD1/1424R |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | IgG, k |
Immunogen | A full length human protein was used as the immunogen for the recombinant Caldesmon antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant Caldesmon antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CALD1 |
UniProt ID | Q05682 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Caldesmon, CDM, CALD1, CAD, CDM Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Titering of the recombinant Caldesmon antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.
IHC testing of FFPE rat uterus with recombinant Caldesmon antibody (clone CALD1/1424R). Required HIER: boil tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min.
IHC-P, WB | |
Human, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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