calbindin D28 Antibody

• Catalog Number: orb1248565
• Category: Antibodies
• Description: calbindin D28 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-KTFVDQYGQRDDGK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 26kDa
• Target: calbindin D28
• UniProt ID: P05937
• NCBI: NP_004920.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CALB1, calbindin 1, 28kDa, CALB, D-28K, OTTHUMP000
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch.Immunohistochemistry:Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248565 (0.03 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248565 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248565 (0.1 ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification.

orb1248565 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.