CA9 Antibody / Carbonic Anhydrase IX

• Catalog Number: orb749854
• Category: Antibodies
• Description: Recognizes a glycoprotein identified as carbonic anhydrase IX (CAIX/gp200). Carbonic Anhydrases (CAs) are members of a large family of zinc metallo-enzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA IX is specifically expressed in clear-cell renal carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CA9/781
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the CAIX antibody.
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CAIX antibody is available for research use only.
• UniProt ID: Q16790
• Storage: Store the CAIX antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CAIX antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with CAIX antibody (CA9/781).

Western blot analysis of HCT116 cell lysate using CAIX antibody (CA9/781). Predicted molecular weight: 50-55 kDa.

SDS-PAGE Analysis of Purified, BSA-Free CAIX Antibody (clone CA9/781). Confirmation of Integrity and Purity of the Antibody.