CA3 Antibody / Carbonic Anhydrase III

• Catalog Number: orb1822870
• Category: Antibodies
• Description: Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. CA III (carbonic anhydrase III), also known as Car3 or CA3, is a 260 amino acid cytoplasmic protein that is specifically expressed in muscle. Belonging to the a-carbonic anhydrase family, CA III is activated by proton donors such as imidazole and dipeptide histidylhistidine, and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CA3/7883
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant fragment of human protein was used as the immunogen for the Carbonic Anhydrase 3 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Carbonic Anhydrase 3 antibody is available for research use only.
• UniProt ID: P07451
• Storage: Aliquot the Carbonic Anhydrase 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human skeletal muscle tissue with Carbonic Anhydrase 3 antibody (clone CA3/7883). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human RH-30 cell lysate with Carbonic Anhydrase 3 antibody (clone CA3/7883). Predicted molecular weight ~29 kDa.

SDS-PAGE analysis of purified, BSA-free Carbonic Anhydrase 3 antibody (clone CA3/7883) as confirmation of integrity and purity.