C5/C5a Antibody

• Catalog Number: orb215941
• Category: Antibodies
• Description: C5/C5a Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat C5a recombinant protein (Position: D1-R77). Rat C5a shares 62% and 82% amino acid (aa) sequences identity with human and mouse C5a, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 8981 MW
• UniProt ID: P08650
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Complement C5;C5a anaphylatoxin;C5;
• Note: For research use only
• Application notes: WB: The detection limit for C5a is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of C5A using anti-C5A antibody.Lane 1:recombinant rat C5A protein 0.5 ng.

WB analysis of C5A using anti-C5A antibody.Lane 1:rat liver tissue.

IHC analysis of C5A using anti-C5A antibody. C5A was detected in a paraffin-embedded section of rat spleen tissue.