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Catalog Number | orb1671603 |
---|---|
Category | Antibodies |
Description | C5AR1 Antibody |
Clonality | Recombinant |
Clone Number | 32-G1 |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | C5AR1 |
UniProt ID | P21730 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | CD88, C5a anaphylatoxin chemotactic receptor, C5a Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody 32-G1. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric r version 32-G1 (orb1671603) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671603- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-C5aR antibody 32-G1. U937 cells were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 32-G1 (orb1671603 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, WB | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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