c-Myc Antibody

• Catalog Number: orb1822814
• Category: Antibodies
• Description: c-Myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MYC/7854R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the c-Myc antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This c-Myc antibody is available for research use only.
• UniProt ID: P01106
• Storage: Aliquot the c-Myc antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil tissue with c-Myc antibody (clone MYC/7854R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cervix tissue with c-Myc antibody (clone MYC/7854R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymphoma with c-Myc antibody (clone MYC/7854R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human K562 cell lysate with c-Myc antibody (clone MYC/7854R). Theoretical molecular weight: ~50 kDa but routinely observed at 50~70 kDa.