c-Kit Antibody / CD117 / SCFR

• Catalog Number: orb1823450
• Category: Antibodies
• Description: This mAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: C117/8399R
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the c-Kit antibody.
• Dilution range: ELISA: 2-4ug/ml (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This c-Kit antibody is available for research use only.
• UniProt ID: P10721
• Storage: Aliquot the c-Kit antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human GIST with c-Kit antibody (clone C117/8399R) at 2 ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free c-Kit antibody (clone C117/8399R) as confirmation of integrity and purity.