c-FER antibody

• Catalog Number: orb1473823
• Category: Antibodies
• Description: Mouse monoclonal antibody to c-FER
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IF, WB
• Reactivity: Mouse
• Immunogen: Recombinant fusion protein of human c-FER. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/2000), IF/IC (1/10 - 1/50), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FER
• Entrez: 14158
• UniProt ID: P70451
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: TYK3; Tyrosine-protein kinase Fer; Feline encephal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of c-FER expression in NIH3T3 (A), mouse testis (B), mouse liver (C) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 100 kD)

Immunofluorescent analysis of c-FER staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of NIH3T3 cells using Anti-c-FER Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.