BUB1 Antibody

• Catalog Number: orb1240079
• Category: Antibodies
• Description: BUB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Bub1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.The immunogen is located within amino acids 40 - 90 of Bub1.
• Concentration: 1 mg/mL
• Dilution range: Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BUB1
• UniProt ID: O43683
• NCBI: O43683
• Storage: Bub1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A
• Note: For research use only
• Application notes: Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 µg/mL.

Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 µg/mL.

Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 µg/mL.