BRSK1 antibody

• Catalog Number: orb1474731
• Category: Antibodies
• Description: Rabbit polyclonal antibody to BRSK1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BRSK1. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: BRSK1
• Entrez: 84446, 381979
• UniProt ID: Q5RJI5, Q8TDC3
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: KIAA1811; SAD1; SADB; Serine/threonine-protein kin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of BRSK1 expression in Hela (A), CT26 (B) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 87 kD)

Immunofluorescent analysis of BRSK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.