Bromodeoxyuridine / BrdU Antibody

• Catalog Number: orb606651
• Category: Antibodies
• Description: This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine / BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BRD2888R
• Tested applications: IHC-P
• Reactivity: All
• Isotype: Rabbit IgG
• Immunogen: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BrdU / Bromodeoxyuridine antibody is available for research use only.
• Storage: Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.
• Expiration Date: 12 months from date of receipt.

IHC staining of BrdU-incorporated FFPE mouse kidney with BrdU antibody (clone BRD2888R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free BrdU antibody (clone BRD2888R) as confirmation of integrity and purity.