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Catalog Number | orb547167 |
---|---|
Category | Antibodies |
Description | BRG1 SMARCA4 Antibody (monoclonal, 3F4) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3F4 |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1 |
Immunogen | E. coli-derived human BRG1 recombinant protein (Position: Q555-E763). |
Concentration | 0 |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 181 kDa |
UniProt ID | P51532 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Application notes | Add 0.2ml of distilled water will yield a concentration of 500μg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U20S cells using anti-BRG1 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of BRG1 using anti-BRG1 antibody.Lane 1:human Raji cell;2:human K562 cell;3:human HL-60 cell;4:human Caco-2 cell;5:human HeLa cell;6:human HepG2 cell;7:rat brain tissue;8:mouse brain tissue;9:mouse lung tissue.
IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human lung cancer tissue.
IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human rectal cancer tissue.
IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of human rectal cancer tissue.
IHC analysis of BRG1 using anti-BRG1 antibody. BRG1 was detected in paraffin-embedded section of rat intestine tissue.
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