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Catalog Number | orb1824831 |
---|---|
Category | Antibodies |
Description | The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2A) and Brg-1 (also designated SNF2B) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/ SNF, including the NURF (nucleosome remodeling factor). |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BRG1/7633R |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the BRG1 antibody. |
Dilution range | ELISA (Order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This BRG1 antibody is available for research use only. |
UniProt ID | P51532 |
Storage | Aliquot the BRG1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human ovarian small cell carcinoma with BRG1 antibody (clone BRG1/7633R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with BRG1 antibody (clone BRG1/7633R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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