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Catalog Number | orb1262785 |
---|---|
Category | Antibodies |
Description | BRD2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Predicted Reactivity | Bovine, Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This BRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human BRD2. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 88 kDa |
Target | BRD2 |
UniProt ID | P25440 |
NCBI | P25440 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Bromodomain-containing protein 2, O2711, Really in Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis in 293 cell line lysates (35 ug/lane).This demonstrates the BRD2 antibody detected the BRD2 protein (arrow).
Western blot analysis of BRD2 using rabbit polyclonal BRD2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRD2 gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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