BRAF Antibody

• Catalog Number: orb1239042
• Category: Antibodies
• Description: BRAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Gallus, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-B-raf antibody (orb1239042) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
• Concentration: 1 mg/mL
• Dilution range: WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 94kDObserved: 94 kD
• Target: BRAF
• UniProt ID: P15056
• NCBI: P15056
• Storage: B-raf antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-o
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Brain Tissue. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1239042, (A: 1 µg/mL; B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: B-raf orb1240089, (0.5 µg/mL), B-raf orb1239042, (0.5 µg/mL) and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken (for orb1240089) and goat anti-rabbit (for orb1239042) IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of B-raf in Human Small Intestine Tissue. Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using anti-B-raf antibody (orb1239042) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of B-raf in Human Small Intestine Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-raf with orb1239042 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).