Brachyury Antibody / Protein T / TBXT

• Catalog Number: orb1823030
• Category: Antibodies
• Description: The T-box gene family consists of members that share a unique DNA binding domain. The best characterized T-box (Tbx) gene, brachyury or T, encodes a transcription factor that plays an important role in early vertebrate development. Tbx genes are a family of developmental regulators with more than 20 members recently identified among invertebrates and vertebrates. Mutations in Tbx genes have been found to cause several human diseases. The understanding of functional mechanisms of Tbx products has come mainly from the prototypical T/brachyury protein, which is a transcription activator. The T-domain is a highly conserved DNA-binding motif originally defined in brachyury and characteristic of the Tbx family of transcription factors. The murine brachyury (T) gene is required in posterior mesoderm formation and axial development. Mutant embryos lacking T gene function are deficient in notochord differentiation and posterior mesoderm formation, but develop anterior mesoderm.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: TBXT/7853R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 235-435) from the human protein was used as the immunogen for the TBXT antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This TBXT antibody is available for research use only.
• UniProt ID: O15178
• Storage: Aliquot the TBXT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human parathyroid tissue with TBXT antibody (clone TBXT/7853R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human chordoma tissue with TBXT antibody (clone TBXT/7853R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.