Bovine IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb346827
• Category: Antibodies
• Description: Bovine IgG (H&L) antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Bovine IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Bovine IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Bovine IgG peroxidase Conjugated Antib
• Note: For research use only
• Application notes: Anti-Bovine IgG Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Purified Rabbit Anti-Bovine IgG Antibody Peroxidase Conjugated tested against purified Bovine IgG HRP. Each well was coated in duplicate with 1.0 µg of Bovine IgG. The working dilution is 1:102000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer, TMB substrate (p/n orb348651).