Bovine IgG F(c) Antibody

• Catalog Number: orb345990
• Category: Proteins
• Description: Bovine IgG F(c) Antibody
• Tested applications: DOT, SDS-PAGE
• Isotype: IgG F(c)
• Concentration: 1.0 mg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c). No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain.
• Conjugation: Unconjugated
• Source: Bovine
• Biological Origin: Bovine
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Bovine IgG F(c) Agarose Conjugate, Bovine IgG Fc A
• Note: For research use only
• Application notes: Bovine IgG F(c) has been tested in SDS-Page and Dot Blot and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Bovine IgG F(c) Fragment. Lane 1: 100 ng Bovine Fc. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: Rabbit Anti-Bovine Fc (p/n orb346822) at 1 µg/ml at RT for 1 hour. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: orb348637 at RT for 30 minutes.

SDS-PAGE for Bovine IgG F(c) Fragment. Lane 1: 1 µg Reduced Bovine Fc. Lane 2: MW ladder Opal pre-stained. Lane 3: 1 µg Non-Reduced Bovine Fc. Coomassie Blue Stained.