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Catalog Number | orb346831 |
---|---|
Category | Antibodies |
Description | Bovine IgG F(c) antibody (Alkaline Phosphatase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine |
Isotype | IgG |
Immunogen | Bovine IgG whole molecule |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine IgG, Bovine Serum and Bovine F(c) and no reaction against Bovine F(ab)2. |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.09% (w/v) Sodium Azide |
Alternative names | rabbit anti-Bovine IgG F(c) alkaline phosphatase C Read more... |
Note | For research use only |
Application notes | Anti-Bovine F(c) Alkaline Phosphatase Conjugate has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. |
Expiration Date | 12 months from date of receipt. |
ELISA Results of Purified Rabbit Anti-Bovine IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Bovine IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(c) (p/n orb345990). The working dilution is 1:5300. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.
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