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Catalog Number | orb345989 |
---|---|
Category | Proteins |
Description | Bovine IgG Antibody |
Tested applications | ELISA, IHC, SDS-PAGE, WB |
Isotype | IgG |
Concentration | 10.0 mg/mL |
Dilution range | ELISA: User Optimized, IHC: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | Bovine IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Bovine IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum and anti-Bovine IgG. |
Conjugation | Unconjugated |
Source | Bovine |
Biological Origin | Bovine |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Bovine IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Bovine Immunoglobulin G; cow IgG Read more... |
Note | For research use only |
Application notes | Bovine IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
Expiration Date | 12 months from date of receipt. |
SDS PAGE Results of Bovine IgG Whole Molecule. Lane 1: Bovine IgG Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Bovine IgG Non-Reduced. 4-20% Gel, Coomassie Stained.
FC, IHC-Fr, WB | |
Bovine, Sheep | |
Monoclonal | |
Unconjugated |
DOT, SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Bovine Serum, anti-Bovine IgG and anti-Bovine IgG F(c). No reaction was observed against anti-Bovine IgG F(ab’)2 or anti-Papain. | |
Bovine |
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