Bovine FGF basic protein

• Catalog Number: orb80023
• Category: Proteins
• Description: Bovine FGF basic protein
• Tested applications: SDS-PAGE
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: Bovine FGF basic
• Solubility (25°C): It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.
• Biological Origin: E.coli
• Biological Activity: The ED50, measured in mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be < 0.1 ng/ml, corresponding to specific activity of 3,000,000 Units/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The FGF-b Bovine was lyophilized from concentrated (1mg/ml) sterile solution containing 1%HSA.
• Alternative names: HBGH-2 protein, HBGF-2 protein, Prostatropin prote
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using calibrated solution of FGF-2 Bovine as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Bovine FGF basic protein