BMP2 Rabbit Polyclonal Antibody

• Catalog Number: orb182463
• Category: Antibodies
• Description: BMP2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BMP2 (341-396/396aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 13/44 kDa
• Target: BMP2
• UniProt ID: P12643
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Song, Liang-Li et al. Expression characteristics of BMP2, BMPR-IA and Noggin in different stages of hair follicle in yak skin Gen Comp Endocrinol, 260, 18-24 (2018)

PubMed: 29174869

Sample: Stomach (Mouse) Lysate at 40 ug, Primary: Anti-BMP2 (orb182463) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/44 kD, Observed band size: 59 kD.

Sample: A431 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-BMP2 (orb182463) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 13/44 kD, Observed band size: 64 kD.

Tissue/Cell: rat stomach tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-BMP2 Polyclonal Antibody, Unconjugated (orb182463) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.