BMI1 Antibody

• Catalog Number: orb97390
• Category: Antibodies
• Description: Mouse monoclonal antibody to BMI1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3E3H3
• Tested applications: FC, ICC, IHC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human BMI1 expressed in E. Coli.
• Antibody Type: Primary Antibody
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Dilution range: IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 37kDa
• Target: BMI1
• Entrez: 648
• UniProt ID: P35226
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Alternative names: PCGF4; RNF51; MGC12685; FLVI2/BMI1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of BMI1 antibody

Western blot analysis of HEK293 (Lane1) and BMI1-hIgGFc transfected HEK293 (Lane2) cell lysate using BMI1 antibody

Confocal immunofluorescence analysis of Hela cells using BMI1 antibody

IHC-P of rectum cancer tissues using BMI1 antibody

Immunohistochemical analysis of formalin fixed and paraffin-embedded cervical cancer tissues using BMI1 antibody

Western blot analysis of human BMI1 (AA: 1-326) recombinant protein using BMI1 antibody

Flow cytometric analysis of NIH/3T3 cells using BMI1 antibody