BMI1 Antibody

• Catalog Number: orb606844
• Category: Antibodies
• Description: The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: BMI1/2689
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A portion of amino acids 142-326 from the human protein was used as the immunogen for the BMI1 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This BMI1 antibody is available for research use only.
• UniProt ID: P35226
• Storage: Store the BMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the BMI1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone BMI1/2689). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone BMI1/2689). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 293T lysate with BMI1 antibody (clone BMI1/2689). Expected molecular weight: 37-43 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Bcl6 antibody (clone BMI1/2689). These results demonstrate the foremost specificity of the BMI1/2689 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free BMI1 antibody (clone BMI1/2689) as confirmation of integrity and purity.