BMI1 Antibody

• Catalog Number: orb1527954
• Category: Antibodies
• Description: BMI1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 276 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: BMI1
• UniProt ID: P35226
• NCBI: NP_005171.4
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: polycomb complex protein BMI-1; flvi-2/bmi-1; RNF5
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human BMI1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, LNCaP, RKO, Hep-G2, U2OS, A-549, Jurkat, K-562, MCF-7, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody orb1527954 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse BMI1 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-BMI1 antibody orb1527954 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human BMI1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BMI1 antibody orb1527954 used for IP at 6 µg/mg lysate.

Detection of human BMI1 by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-BMI1 (Cat. No. orb1527954 -left and Cat. No. orb1527954 -right) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB