BLK Antibody

• Catalog Number: orb1249224
• Category: Antibodies
• Description: BLK Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: QWSPLKVSAQDKD-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BLK
• UniProt ID: P51451
• NCBI: NP_001706.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: BLK, B lymphoid tyrosine kinase, MGC10442, MODY11,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in Human Thymus, Spleen and Lymph Node lysates (calculated MW of 57.7kDa according to NP_001706.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249224 (0.1 ug/ml) staining of Human Thymus lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249224 (3.75 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.