BIVM antibody

• Catalog Number: orb750471
• Category: Antibodies
• Description: BIVM antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: BIVM peptide corresponding to a region near carboxy terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 90 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by delipidation and defibrination. This polyclonal antibody reacts with BIVM. This conserved protein is present in many tissues and organisms and therefore cross-reactivity with BIVM from other sources is likely but has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q86UB2
• NCBI: NP_001153068.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-BIVM Antibody, Basic immunoglobulin-li
• Note: For research use only
• Application notes: This polyclonal antibody reacts human BIVM at the C-terminal region and is useful for detection of BIVM by ELISA or western blotting. For immunoblotting a 57 kDa band corresponding to human BIVM is detected. Unstimulated HeLa cells can be used as a positive control. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
• Expiration Date: 12 months from date of receipt.

Western blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kDa band corresponding to human BIVM protein is detected using a 1:1000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) code orb347654 diluted 1:10000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure). Other detection methods will yield similar results.