Biotinylated IL-22 Recombinant Protein

• Catalog Number: orb1231478
• Category: Proteins
• Description: Biotinylated IL-22 Recombinant Protein
• Tested applications: ELISA, WB
• Tag: His Tag & Avi Tag
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE.
• MW: 20.3 kDa
• Protein Sequence: Ala 34 - Ile 179
• Source: HEK293 cells
• Storage: Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: IL-22,IL-TIF,ZCYTO18
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag). The protein has a calculated MW of 20.3 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Biotinylated Human IL-22, His, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Immobilized Biotinylated Human IL-22, His, Avitag at 1 ug/ml (100 uL/well) on streptavidin precoated (0.5 ug/well) plate. can bind Human IL-22 Antibody with a linear range of 0.1-4 ng/mL.

Immobilized Human IL-22 Antibody at 1 ug/ml (100 uL/well) can bind Biotinylated Human IL-22, His, Avitag with a linear range of 1-31 ng/mL.