Biotinylated Human Complement C3 Protein

• Catalog Number: orb1176546
• Category: Proteins
• Description: Biotinylated Human Complement C3, His, is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
• Reactivity: Human
• Tag: C-10×His & C-Avi
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Biotin
• MW: 188.5 kDa
• Target: Complement C3
• UniProt ID: P01024
• Source: Biotinylated Human Complement C3, His, (orb1176546) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Asn 1663 (Accession # P01024-1).
• Expression System: HEK293
• Biological Origin: Human
• Biological Activity: Immobilized Biotinylated Human Complement C3, His, at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-C3,Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: Ser 23 - Asn 1663
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000055.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C3,CPAMD1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (). The protein has a calculated MW of 188.5 kDa. The protein migrates as 72 kDa,120-125 kDa and 200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human Complement C3, His, Avitag (Cat. No. orb1176546) at 1 μg/mL (100 μL/well)on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-C3, Human IgG1 Human Kappa with a linear range of 0.1-4 ng/mL (QC tested).