Biotin / Vitamin B7 / Vitamin H Antibody

• Catalog Number: orb606472
• Category: Antibodies
• Description: This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BTN/2032R
• Tested applications: IHC-P
• Isotype: Rabbit IgG, kappa
• Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Biotin antibody is available for research use only.
• UniProt ID: Not Applicable
• Storage: Store the recombinant Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Kappa Light Chain Ab probe followed by anti-Biotin antibody (clone BTN/2032R). No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

SDS-PAGE analysis of purified, BSA-free recombinant Biotin antibody (clone BTN/2032R) as confirmation of integrity and purity.