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Catalog Number | orb1826354 |
---|---|
Category | Antibodies |
Description | This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BTN/8672R |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | All |
Isotype | Rabbit IgG, kappa |
Immunogen | Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT |
Conjugation | Unconjugated |
Formula | 1 mg/ml in 1X PBS; BSA free, sodium azide free |
Hazard Information | This Biotin antibody is available for research use only. |
Storage | Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human kidney tissue with Biotin antibody (clone BTN/8672R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
FACS, IF, IHC-P, WB | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P | |
Rabbit | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
All | |
Mouse | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
All | |
Mouse | |
Recombinant | |
Unconjugated |
FACS, IF, IHC-P, WB | |
All | |
Mouse | |
Recombinant | |
Unconjugated |
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