Biotin / Vitamin B7 / Vitamin H Antibody

• Catalog Number: orb1822659
• Category: Antibodies
• Description: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rBTN/8819
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: All
• Isotype: Mouse IgG1, kappa
• Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Biotin antibody is available for research use only.
• Storage: Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human kidney tissue with biotinylated Lambda Light Chain antibody probe followed by Biotin antibody (clone rBTN/8819).

SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone rBTN/8819) as confirmation of integrity and purity.