You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb533181 |
---|---|
Category | Tools |
Description | Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid) |
SMILES | OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]4SC[C@@H]5NC(=O)N[C@H]45)ncnc23)C[C@@H]1O |
Formula | C32H54N9O15P3S |
Hazard Information | Non-Toxic |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. < br>< b>Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
Expiration Date | 12 months from date of receipt. |
Structural formula of Biotin-14-dATP.