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Catalog Number | orb334487 |
---|---|
Category | Antibodies |
Description | Bik Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Bik recombinant protein (Position: M1-R123). Human Bik shares 42.3% amino acid (aa) sequence identity with mouse Bik. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 18016 MW |
UniProt ID | Q13323 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Bcl-2-interacting killer;Apoptosis inducer NBK;BIP Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of MCF-7 cells using anti-BIK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of THP-1 cells using anti-BIK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
Flow Cytometry analysis of A431 cells using anti-BIK antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Bik using anti-Bik antibody.Lane 1:Rat Spleen Tissue;2:Rat Gaster Tissue;3:Rat Intestine Tissue;4:MCF-7 Cell;5:A549 Cell;6:SKOV Cell.
IHC analysis of Bik using anti-Bik antibody.Bik was detected in paraffin-embedded section of Mouse Spleen Tissue.
IHC analysis of Bik using anti-Bik antibody.Bik was detected in paraffin-embedded section of Rat Spleen Tissue.
IHC analysis of Bik using anti-Bik antibody.Bik was detected in paraffin-embedded section of Human Thyroid Cancer Tissue.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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