BHMT Antibody

• Catalog Number: orb1246747
• Category: Antibodies
• Description: BHMT Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-EQQLKELFEKQK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BHMT
• UniProt ID: Q93088
• NCBI: NP_001704.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: BHMT, betaine-homocysteine methyltransferase
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246747 (0.03 ug/ml) staining of Rat Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

HEK293 overexpressing BHMT and probed with orb1246747 (mock transfection in first lane).

orb1246747 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246747 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.