Beta Tubulin Antibody / TUBB3

• Catalog Number: orb1825229
• Category: Antibodies
• Description: This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rTUBB3/7405
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 250-450) from the human protein was used as the immunogen for the recombinant TUBB3 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This recombinant TUBB3 antibody is available for research use only.
• UniProt ID: Q13509
• Storage: Aliquot the recombinant TUBB3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human brain tissue with recombinant TUBB3 antibody (clone rTUBB3/7405). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant TUBB3 antibody (clone rTUBB3/7405) as confirmation of integrity and purity.

Western blot testing of human PC3 cell lysate with recombinant TUBB3 antibody (clone rTUBB3/7405). Predicted molecular weight ~50 kDa.