Beta Catenin Antibody

• Catalog Number: orb533983
• Category: Antibodies
• Description: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CTNB1-1R
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Beta Catenin protein was used as the immunogen for the recombinant beta Catenin antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Prediluted IHC only format : incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant beta Catenin antibody is available for research use only.
• UniProt ID: P35222
• Storage: Store the recombinant beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the recombinant beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human HeLa cells with recombinant Beta Catenin antibody (clone CTNB1-1R, green) and Reddot nuclear stain (red).

Western blot testing of human HeLa antibody with recombinant Beta Catenin antibody (clone CTNB1-1R). Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.

IHC staining of FFPE human breast cancer tissue with recombinant beta Catenin antibody (clone CTNB1-1R). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.