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Catalog Number | orb1294385 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Beta Catenin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rabbit |
Isotype | Rabbit-IgG |
Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
Dilution range | Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:100-1:300 |
Conjugation | Unconjugated |
Target | Beta-catenin |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-Beta Catenin antibody at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294385 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling Beta Catenin with orb1294385 at 1/200.
Immunohistochemical analysis of paraffin embedded Human HER2+ Breast carcinoma tissue labeling Beta Catenin with orb1294385 at 1/200.
Immunohistochemical analysis of paraffin embedded Human HER2+ Breast carcinoma tissue labeling Beta Catenin with orb1294385 at 1/200.
Anti-Beta Catenin antibody at 1/1000 dilution, Lysates at 40 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294385 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294385 anti-Beta Catenin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Application: FFPE immunohistochemistry; Condition/Dilution 1-200; Epitope Retrieval methods: Citrate Buffer, pH 6.0; Sample: Rat skin.
Application: FFPE immunohistochemistry; Condition/Dilution 1-200; Epitope Retrieval methods: Citrate Buffer, pH 6.0; Sample: Rat skin.
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, IHC-P | |
Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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