Beta Amylase antibody (Biotin)

• Catalog Number: orb344948
• Category: Antibodies
• Description: Beta Amylase antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Plant
• Isotype: IgG
• Immunogen: Beta Amylase [Sweet Potato]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]. Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P10537
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Beta Amylase Antibody biotin Conjugati
• Note: For research use only
• Application notes: Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Dot Blot results of Rabbit Anti-Beta Amylase Biotin Conjugated. Antigen: Beta Amylase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta Amylase Biotin 1 µg/mL for 1 hr at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT.