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Beta Amylase Antibody Biotin Conjugated
Catalog Number: orb344947
| Catalog Number | orb344947 |
|---|---|
| Category | Antibodies |
| Description | Beta Amylase antibody (Biotin) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | DOT, ELISA, WB |
| Reactivity | Plant |
| Isotype | IgG |
| Immunogen | Beta Amylase [Sweet Potato] |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000 |
| Form/Appearance | Lyophilized |
| Purity | Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato]. Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined. |
| Conjugation | Biotin |
| UniProt ID | P10537 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Alternative names | rabbit anti-Beta Amylase Antibody biotin Conjugati Read more... |
| Note | For research use only |
| Application notes | Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Expiration Date | 12 months from date of receipt. |
Dot Blot results of Rabbit Anti-Beta Amylase Biotin Conjugated. Antigen: Beta Amylase. Blot loaded at 3 fold dilution: 1. 100 ng, 2. 33.3 ng, 3. 11.1 ng, 4. 3.70 ng, 5. 1.23 ng. Blocking: orb348637 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta Amylase Biotin 1 µg/ml for 1hr at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT.
