beta Actin antibody

• Catalog Number: orb181785
• Category: Antibodies
• Description: beta Actin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant human beta actin protein (orb84841) was used as immunogen.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 1:500, IHC: 1:200, IF/ICC: 1:200
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: beta Actin
• UniProt ID: P60709, P60710
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ACTB antibody, anti Actin cytoplasmic 1 antib
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Li Xu 1, Jie Su 2, Caili Du 3, Hui Liu 4, Wei Lv Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3 Cell Mol Biol (Noisy-le-grand), (2024)

PubMed: 38158667

Applications

IF

Reactivity

Rat

Wang, Weiwei et al. Notoginsenoside R1 alleviates high glucose-evoked damage in RSC96 cells through down-regulation of miR-503 Artif Cells Nanomed Biotechnol, 47, 3947-3954 (2019)

PubMed: 31581849

Immunohistochemical staining of paraffin embedded mouse brain tissue using beta actin antibody (primary antibody at 1:200)

IHC-P staining of mouse brain tissue using anti-beta actin (dilution at 1:200)

WB analysis of recombinant human beta actin antibody (dilution of 1:500)

Immunofluorescence analysis of mouse brain tissue using beta actin antibody (dilution of primary antibody - 1:200)

IF analysis of mouse brain tissue using beta actin antibody (dilution of primary antibody at 1:200)

IHC-P image of mouse brain tissue using anti-beta actin (dilution of primary antibody at 1:200)

Immunohistochemical staining of human breast cancer tissue using beta actin antibody (dilution of primary antibody - 1:200)