Beta-actin Antibody

• Catalog Number: orb1294415
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Beta Actin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Dilution range: Western Blot 1:2000-1:5000 Immunofluorescence 1:600-1:700 Immunohistochemistry (Paraffin) 1:400-1:600
• Conjugation: Unconjugated
• Target: Beta-actin
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-Beta Actin antibody - at 1/2000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294415 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with orb1294415 at 1/600.

Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 Anti-Beta Actin antibody (red) at 1:400 and 4°C.

Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 Anti-Beta Actin antibody (red) at 1:400 and 4°C.

Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 Anti-Beta Actin antibody (red) at 1:400 and 4°C.

Immunofluorescence: cells were fixed with 4% PFAfor 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294415 Anti-Beta Actin antibody (red) at 1:400 and 4°C.