beta Actin antibody

• Catalog Number: orb10033
• Category: Antibodies
• Description: beta Actin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human beta Actin. Please contact us for the exact immunogen sequence. The peptide is available as orb374699.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml)
• Form/Appearance: C: 10 mM PBS, 0.02% Sodium Azide, 10 mg/ml rAlbumin, 50% Glycerol
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: beta Actin
• Entrez: 60
• UniProt ID: P60710, P60711, P60709
• NCBI: 31, 001101, 001092, 11
• RRID: AB_10749201
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: anti ACTB antibody, anti Actin cytoplasmic 1 antib
• Note: For research use only
• Application notes: This Beta Actin antibody is against Human, Mouse, Rat, cat, Chicken, Cow, Dog, Fish, Guinea Pig, hm, mMouset, op, Pig, Rabbit, Sheep.
• Expiration Date: 12 months from date of receipt.

References

Faria, R. S. et al. Classical aversive conditioning induces increased expression of mature-BDNF in the hippocampus and amygdala of pigeons Neuroscience, 255, 122-133 (2013)

PubMed: 24121128

Applications

WB

Reactivity

Animal

Zhu, Yuxuan et al. Antioxidant and antiapoptotic effects of 1,1'-(biphenyl-4,4'-diyl)-bis(3-(dimethylamino)-propan-1-one) on protecting PC12 cells from Aβ-induced injury Mol Pharm, 11, 428-435 (2014)

PubMed: 24350730

Applications

WB

Reactivity

Human

Western blot analysis of rat lung lysates using Beta Actin antibody

Western blot analysis of mouse brain lysate using Beta Actin antibody

Immunohistochemical staining of paraffin embedded rat renal glomerulus tissue stained with Beta Actin antibody

Immunohistochemical staining of paraffin embedded rat renal glomerulus tissue stained with Beta Actin antibody

Immunohistochemical staining of paraffin embedded rat renal glomerulus tissue stained with Beta Actin antibody (DAB as chromogen)