Beta-2 Microglobulin Antibody

• Catalog Number: orb750143
• Category: Antibodies
• Description: Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MGBP2-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the B2M antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This B2M antibody is available for research use only.
• UniProt ID: P61769
• Storage: Store the B2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the B2M antibody may be required for optimal performance.> 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human renal carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human bladder carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human melanoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Immunofluorescent staining of human HeLa cells with B2M antibody (green, clone MGBP2-1) and Reddot nuclear stain (red).

Flow cytometry testing of human HeLa cells with B2M antibody (clone MGBP2-1); Red=isotype control, Blue= B2M antibody.

Western blot testing of human Raji cell lysate with B2M antibody (clone MGBP2-1).