Beta-2 Microglobulin Antibody

• Catalog Number: orb749756
• Category: Antibodies
• Description: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B2M/961
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the Beta-2 Microglobulin antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Beta-2 Microglobulin antibody is available for research use only.
• UniProt ID: P61769
• Storage: Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human renal carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).

Immunofluorescent staining of human HeLa cells with Beta-2 Microglobulin antibody (green, clone B2M/961) and Reddot nuclear stain (red).

Western blot testing of human Raji cell lysate with Beta-2-Microglobulin antibody (clone B2M/961).

Flow cytometry testing of human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/961); Red=isotype control, Blue=Beta-2 Microglobulin antibody.

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/961).