Beta-2 Microglobulin Antibody

• Catalog Number: orb749753
• Category: Antibodies
• Description: Serum Beta-2 Microglobulin (B2M) levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM617
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the B2M antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This B2M antibody is available for research use only.
• UniProt ID: P61769
• Storage: Store the B2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the B2M antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of permeabilized human HeLa cells with B2M antibody (clone SPM617).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with B2M antibody (clone SPM617).

Flow cytometry testing of PFA-fixed human HeLa cells with B2M antibody (clone SPM617); Red=isotype control, Blue=B2M antibody.

Western blot testing of human 1) ThP-1 and 2) Raji cell lysate with B2M antibody (clone SPM617). Expected molecular weight: 12-14 kDa.