Beta-2 Microglobulin Antibody

• Catalog Number: orb749752
• Category: Antibodies
• Description: Beta-2 microglobulin is a 12KDa protein with a pI of 5.6. Serum beta2 microglobulin levels are a reflection of cell turnover. Levels rise with fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin's lymphoma. In urine, increased levels are seen in proximal renal tubular disease as well as renal transplant rejection. Beta-2 microglobulin levels can rise either because its rate of synthesis has increased (e.g. in AIDS, malignant monoclonal plasma cell dyscrasia, solid tumours and autoimmune disease) or because of impaired renal filtration (e.g. due to renal insufficiency, graft rejection or nephrotoxicity induced by post-transplantation immunosuppressive therapy).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B2M/1118
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length recombinant human B2M protein was used as the immunogen for the Beta-2 Microglobulin antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Beta-2 Microglobulin antibody is available for research use only.
• UniProt ID: P61769
• Storage: Store the Beta-2 Microglobulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Beta-2 Microglobulin antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Beta-2-Microglobulin antibody (clone B2M/1118).

Immunofluorescent staining of permeabilized human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/1118).

Western blot testing of human THP-1 and Raji cell lysate with Beta-2 Microglobulin antibody. Expected molecular weight: 12-14 kDa.

Flow cytometry testing of permeabilized human HeLa cells with Beta-2 Microglobulin antibody (clone B2M/1118); Red=isotype control, Blue=Beta-2 Microglobulin antibody.

SDS-PAGE analysis of purified, BSA-free Beta-2 Microglobulin antibody (clone B2M/1118) as confirmation of integrity and purity.