Beta-2 Microglobulin Antibody

• Catalog Number: orb639506
• Category: Antibodies
• Description: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alpha heavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alpha heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alpha heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: B2M/1857R
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant B2M antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant B2M antibody is available for research use only.
• UniProt ID: P61769
• Storage: Store the recombinant B2M antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant B2M antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human bladder carcinoma stained with recombinant B2M antibody (B2M/1857R). Required HEIR: boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant B2M antibody (clone B2M/1857R); Red=isotype control, Blue=recombinant B2M antibody.

Western blot testing of human Raji cell lysate with recombinant B2M antibody (clone B2M/1857R). Expected molecular weight: 12-14 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant B2M antibody (clone B2M/1857R) as confirmation of integrity and purity.

Immunofluorescent staining of PFA-fixed human HeLa cells with recombinant B2M antibody (clone B2M/1857R).